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). In the cost-free ChVLig-AMP, the OB area is mirrored faraway from the NTase area to fully expose the DNA-binding surface above the AMP-binding pocket. The peptide phase that is certainly destined to become the latch is disordered during the free of charge ligase and delicate to proteolysis, but this segment is shielded from proteolysis when ChVLig binds to nicked DNA.
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